Automated GPC/SPE-freestyle is used in analytical chemistry to perform the process of purifying and separating macromolecules such as polymers, proteins, and nucleic acids based on their size. Solid phase extraction, also known as SPE, is a method for isolating and concentrating target analytes from a complicated sample matrix. Automated GPC/SPE-freestyle system enables automated sample preparation while combining the advantages of both approaches. Depending on the kind of sample and the target analytes, the system can be programmed to carry out particular separation and purification stages. A GPC column is used to separate macromolecules in the sample depending on their size.