Using high performance liquid chromatography (HPLC) systems, it is possible to separate, recognise, and quantify the constituent parts of complicated mixtures. A mobile phase, stationary phase, pump, detector, and injection system are some of the parts of an HPLC system. The stationary phase, which is often a solid or liquid substance that offers a separation mechanism, is typically a solvent or combination of solvents that transports the sample through it. The injection mechanism enters the sample into the mobile phase, which is being continuously pumped out by the pump. High performance liquid chromatography (HPLC) systems have a detector that recognises the separated components.