Immunoaffinity column for aflatoxin is a form of chromatography column that uses antibodies to capture and extract particular molecules out of a complicated mixture. When it comes to aflatoxin, an IAC can be utilised to capture and isolate the toxin from a sample in a targeted manner. A considerable risk to both human and animal health exists when certain moulds develop aflatoxins, which are harmful by-products that can contaminate food and feed items. A sample must first be prepared by removing the toxin from the sample matrix using the proper solvent in order to employ an immunoaffinity column for aflatoxin.