Automated GPC/SPE refers to the employment of automated technologies to carry out both the solid-phase extraction (SPE) and gel permeation chromatography (GPC) operations in a single workflow. Based on their size, GPC is a separation technique used to analyse macromolecules like polymers, proteins, and peptides. For the extraction and purification of target analytes from complex matrices, such as biological samples and environmental samples, SPE is a sample preparation technique that is used. Automated GPC/SPE is employed in several industries, including forensic investigation, pharmaceuticals, food and beverage, and environmental analysis. It is helpful when analysing complicated samples that comprise numerous analytes with varying characteristics, such as size, polarity, and hydrophobicity.